Urethral stricture formation is caused by fibrosis after excessive collagen formation to the any injury or trauma to the urethra. We evaluated the affects of platelet-rich plasma (PRP) on urethral injury model of male rats with bladder findings.
Urethral injury model was performed by coagulation current to the male urethra. There were 4 groups of 6 rats in each one: control (C), C+PRP applied, urethral injury (UI), UI+PRP applied. PRP was applied to the urethra after coagulation current induced injury as soon as possible. On 14th day, infravesical obstruction model was obtained which was shown with retrograde urethrogram. On 14th day all rats were sacrified and bladder tissues investigated for collagen type I (col I), collagen type III (col III), platelet-derived growth factor-A (PDGF-A), platelet-derived growth factor-B (PDGF-B) and transforming growth factor-B (TGF-B) with Western-Blot analysis. The effect of damage and healing was investigated for col I/col III ratio.
Collagen type I and collagen type III increase in fibrosis process in UI is well defined. Collagen type I/ collagen type III ratio was significantly high in UI group (**p<0.01) than others in Western Blot analysis (Graphic 1 and 2) moreover col I/COL III ratio was significantly lower in UI+PRP group than UI group which was not significant and similar with C+PRP applied group. Western Blot analysis of col I, col III, PDGF-A, PDGF-B and TGF-B was shown in table 1.
Our results show that PRP has a preventive effect on bladder tissue in UI as a rat model of infravesical obstruction. Further studies that eventually show the effects of PRP on animal and human tissues is necessary and promising.