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447: Whole genome epigenetic characteristics of pelvic lipomatosis: The new hope to prevent and cure this disease

Speaker
G. Xiong, Beijing (CN)
Authors
Xiong G., He S., Li X., Han W., Zhou L.
Institutions
Peking University First Hospital, Dept. of Urology, Beijing, China, 0
Event
33rd Annual EAU Congress Copenhagen
Date – time - Location
18 March 2018, 12:15 - 13:45, Green Area, Room 1 (Level 0)
Session
Poster Session 33 - Better understanding LUTS: A look behind the curtain
Topic
Functional LUTS, incontinence and neuro-urology: Basic science

Introduction & Objectives

Pelvic lipomatosis (PL) is a disorder of benign mature adipose tissue proliferation around bladder, ureter and rectum, there is no effective way to cure the recurrent LUTS symptoms for the PL patients completely. The aetiology of this disease is not well understood, we conducted this study to identify gene methylation status of pelvic lipomatosis adipose tissue.

Materials & Methods

Using the methylation sequencing technology, we analyzed and compared the whole genome methylation status of abnormal pelvic adipose tissue and subcutaneous fat tissue from the same patient.

Results

Pelvic lipomatosis present malignant-like whole genome epigenetic characteristics: Abnormal adipose tissue presented lower methylation in the whole genomic level, however, the methylation level were significantly higher in the promoter, utr5 and exon areas. There were significant differences in DMR (Differentially Methylated Region) and DMP (Differentially Methylated Promoter) between abnormal and normal adipose tissue. After enrichment analysis using Gene Ontology (GO) database, we identified several GO terms related to cell adhesion (cell adhesion, cell-cell adhesion, biological adhesion, membrane, binding, cell-cell adhesion via plasma-membrane adhesion molecules, et al. All p<0.001), this could be a good explanation of the pelvic adipose tissue adhesion phenomenon in the operation. Using KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis, we have confirmed a series of significant pathways. Pathogenic Escherichia coli infection (p<0.001), Bacterial invasion of epithelial cells (p=0.010), Nicotine addiction (p=0.002) and cell adhesion molecules (p=0.007) might have significant clinical value.

Conclusions

Compared with normal subcutaneous fat tissue, abnormal pelvic adipose tissue of PL patients presented significant changes in the whole genome methylation level. Smoking cessation and prevention of urinary tract infection might benefit PL patients, and methylation regulation drugs might be a new research direction to the treatment of this disease.